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Bacteriophage Capture and Release from Yeast

Bacteriophage capture and release from Yeast

Instructor: Paul Jaske, PhD

In this class we will learn about the incredible world of bacteriophages: tiny little viruses that hunt bacterial cells rather than animals. You're probably wondering: what do yeast and bacteriophages have in common? The answer is absolutely nothing, until we put them together! Using a recently developed technique we will commandeer the amazing natural ability of yeast to stitch pieces of DNA together to 'capture' a bacteriophage genome inside yeast and then 'release' it later into its natural host, E. coli.

In this class you will learn about:
• Yeast and its homologous recombination system (DNA stitching machinery)
• Yeast plasmids
• Bacteriophage (the little viruses that hunt bacteria)
• PCR primer design

After taking this class you will know how to:
• Make yeast competent (able to take up DNA)
• Transform yeast
• Design pieces of DNA that can be stitched together
• Cut DNA apart with restriction enzymes
• Join (ligate) separate pieces of DNA back together again
• Transform E. coli
• Infect E. coli with bacteriophage


At least two other BioCurious lab classes, one being transforming bacteria with pGLO (aka Make Cells Glow with GFP). Please let us know if you have any questions about the pre-requisites.


Sunday June 17th 1pm - 5pm

Wednesday June 20th 6:30pm - 8:30pm

Cost: $50

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  • Paul J.

    Great class everybody! I'll be posting pictures of the results in a couple days.

    July 3, 2012

  • Paul J.


    June 20, 2012

  • Anitha B.

    So, the second part of this class is actually on Wednesday, June 27th, 2012 from 6:30PM to 8:30PM, not today.

    June 20, 2012

  • Tieniu L.

    My son Alan did not take the prereqs, so we'll just come and see if he could join.

    June 17, 2012

  • Anitha B.

    I'm signed up for this, just not on Hope to see everyone there!

    June 16, 2012

  • Nav

    Yes, i've used micro pipettes in a number of places including a Schmall science class on the Heritage Rose Garden flowers.

    June 16, 2012

  • Maria C.

    Nav: What sort of lab experience do you have? Are you familiar with using a micro pipette etc?

    June 16, 2012

  • Nav

    So I've previously worked with pGLO and bioluminescence in a Biology AP class 3 years ago. I have not taken any of these lab classes with BioCurious lab however. Can I still register for this class?

    June 16, 2012

  • Paul J.

    Not sure about that. I know we had been offering a bunch during the winter and spring but it might have slowed down a bit recently.

    June 11, 2012

  • arjun

    Are the necessary prerequisites being offered anytime in the near future?

    June 11, 2012

8 went

Your organizer's refund policy for Bacteriophage Capture and Release from Yeast

Refunds are not offered for this Meetup.

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