BioCurious Message Board › Some results from last week's lab

Some results from last week's lab

Eri G.
supersalad
Group Organizer
Palo Alto, CA
Pics and results posted on http://biocurious.org...­

Feel free to add to the discussion on the wiki

- Eri
A former member
Post #: 7
Great pics and discussion but one "not" was missing, I think, somewhere after "Unfortunately".
Peter
user 11892350
Boston, MA
Post #: 1
Took a look at your experimental conditions (on the whiteboard pic), and noticed you guys are using combinations of glycerol/DMSO/glycol in your different test solutions. Just wondering if you guys are also trying recombinant approaches, for example E. coli strains engineered (possibly by genomic integration) to express antifreeze proteins:

http://www.springerli...­
http://tiny.cc/4mbcv...­

That might be an interesting project -- a DIY-engineered E.coli strain that is amenable to storage at -20C for prolonged times, that can be distributed to DIYbio communities globally.


Also, trehalose has been shown to be superior to DMSO or glycerol for cryostorage of cells:
http://www.springerli...­
http://www.nature.com...­
http://www.ncbi.nlm.n...­

In addition, trehalose is dirt cheap, much cheaper than glycerol or DMSO; it goes for about $5-10 per pound:

http://www.swansonvit...­


A former member
Post #: 14
does this mean the experiment will be repeated? I'm back in town for a few weeks and eager to get involved.
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