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DIY Molecular Cloning: Plasmid Construction

  • May 27, 2014 · 7:00 PM
  • Ahnon's

So, you survived a introductory lecture and a banana cloning experiment. Now, let's get serious about Cloning.

I've spent the last couple of days revamping the class so we can perform high quality molecular bio experiments. I've created a syllabus that can be found at https://counterculturelabs.org/?page_id=219

Within the syllabus is the course budget. That budget can be validated online if you desire. I would ask that if you decide to attend the class, that you pitch in with the cost of reagents.

You will also find the class dates and topics to be discussed with assigned reading materials. We will cover specific topics each class (Every other Tuesday starting March 4, 2014) and perform corresponding laboratory work in the class. Lectures will be more focused and occur before lab work or during experimental wait times.

Today's class topic will be Plasmids Structure and Function, and we will be doing some digests and ligations in the lab.

I intend to have you leave the class in June with a thorough understanding of how to perform your own cloning experiments!

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