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Lab Working Bee Thursday March 28th at 6PM

A community lab does not maketh itself. We need your help, as part of our growing DIYbio community, to help us catalog and set up all the equipment which has been generously donated by our Fairy God Parents. So roll up your sleeves and join us on March 28th at 2351 Faraday Ave, Carlsbad 92008 to get involved. The best way to learn exactly what we have and what experiments you could do in our space is to come and help set it all up! Also, use this chance to talk to San Diego's DIYbio crowd about how you can use our community lab to advance your project or learn more about science.

Bring your project ideas and have them ready to record on camera, because, that's right, we will be recording them!

Sign up on biotechnbeyond.com/join and describe your project briefly. In the Subject line start with "My project" then an apt short name for your project. In the box below the subject line describe what your project is in 2 very short sentences. This video will be for our crowdfunding campaign to raise money for the laboratory. In essence, you will be raising awareness for your own research/product and helping us fund the lab at the same time!

I recommend bringing a diagram or a small prop to help describe your ideas as it may become part of our 5 minute crowdfunding video. We will be shooting more film later this week and the following week so if you can't make it this Thursday contact me either through meetup or by email at:  sportsman dot rich at gmail dot com

Again be mindful we can't put everyone's project into a 5 minute video (although we will certainly try!) but if you can prepare 2 minutes of talking points about your project we will try to get a brief synopsis of as many projects as we can!

Dilanka has graciously agreed to help film and I will be setting up some audio recording. The hope is to have some interviews about possible projects, so if you would like to get involved in our video (which will be on a crowdfunding site near your internet accessible device soon) please let us know, but we will not release any footage of you without your permission!

Kevin, Babak, and Richard Rouse will be organizing the equipment list. Max has already done some of the work for us (thank you Max!) but we need to get some more information from each piece of equipment especially the energy requirements for each unit. So come on down to the Beyond, we will provide refreshments and a good time to meet other fellow citizen scientists!


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  • Helge Z.

    Great to be there tonight and meet some of you.

    Here is a list of equipment required for most molecular biology projects (cloning and bacterial strain construction work).

    Set of pipettors for[masked] ul volumes, including multichannels (8- and 12-channel)
    Vortexer
    Heat blocks
    PCR machine with 96-well block
    Incubator, 37 °C
    Incubator, variable temp
    Microshaker, variable temp
    Clinical centrifuge with adaptors for 15 ml tubes, 50ml tubes and plates
    Microcentrifuge
    Speedvac (for plates)
    Agarose gel running equipment (gel boxes and power supplies)
    Gel imaging system
    Dark reader (blue light)
    Fluorometer (for measuring DNA concentrations)
    Electroporator
    Water bath, variable temp
    Autoclave

    I am happy to answer any molecular biology related questions that anyone may have.
    Cheers,
    Helge

    March 29, 2013

  • r3sh

    I'm on my way. Running a little late. Lot of traffic.

    March 28, 2013

  • carol

    Can't make it this week.
    Is there a way I can donate some cash to hire someone to help taking up the floor tiles? Or maybe I can hire someone to take up the tiles.

    March 27, 2013

  • Tom H.

    I live in North Park and have never attempted to drive from here to Carlsbad at the height of rush hour, but I think most of the traffic jams are in the opposite direction.

    March 19, 2013

    • Richard R.

      its not so bad after you get past del mar hell

      March 27, 2013

    • Leah C.

      And there's always the 101 which is like driving through holiday towns!

      March 27, 2013

  • Richard S.

    Hey Guys so Leah summed it all up for us very well. Thanks Leah!

    But another note is if you guys can all sign up for the Bulletin on our website biotechnbeyond.com/join
    in the Subject line start with "My project" then an apt short name for your project. In the box below describe briefly what your project is about in 2 short sentences!

    We will also be filming some video in the lab. Dilanka has graciously agree to help film and I will be setting up some audio recording. The hope is to have some interviews about possible projects, so if you would like to get involved in our video (which will be on a crowdfunding site near your internet accessible device soon) please let us know but we will not release any footage of you without your permission!

    Kevin, Babak, and Richard Rouse will be organizing the equipment list. Max has already done some of the work for us but we need to get some more information from each piece of equipment especially the energy requirements for each unit.

    March 25, 2013

  • Leah C.

    So we have 3 tasks we need to get done Thursday night:
    1) Itemize all equipment using a spreadsheet template we have that lists serial #, plug type, etc. We will provide hard copies of this and also use laptops (since we finally have internet)!

    2) Richard Sportsman will be compiling project descriptions, so if you have a project you want to start in the lab (hopefully everyone does!), sit down with him for 10 mins or so and get the research proposal submitted. 3) Set up the existing computers.

    See you there!

    March 25, 2013

  • Max B.

    I made a partial list of equipment last time I was there - see https://docs.google.com/spreadsheet/ccc?key=0AhzFJmuDxgnJdEdERzJPWllMT24xcE9WaC1yalViUEE&usp=sharing

    It's got pretty much all the big equipment in the garage, and some of the stuff around the benches. Still missing the various fridges/-20s, as well as the more custom and esoteric equipment.

    March 21, 2013

  • Katrina

    Do you happen to have a list of equipment?
    Look forward :)

    March 20, 2013

    • Leah C.

      Not an up to date list yet - that is part of the goals for the working bee. I'll try and get a draft list posted this weekend.

      March 20, 2013

  • George F.

    this will be fun, we'll find out what we have and what we still need.

    March 20, 2013

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