Microbe Hunt: Identification with 16S rRNA Sequencing

This is a past event

5 people went

Price: $30.00 /per person
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We've successfully found and isolated some colorful microbes, but now we need to identify them! While there are several ways to go about this, the quickest and easiest is to sequence their DNA. Join us for this two-part class to learn all the steps involved in identifying a bacterial specimen through DNA sequencing.

Part 1, March 7 at 7pm: We'll prepare our bacterial isolates for sequencing by streaking them onto fresh agar plates. You'll learn aseptic techniques for working with bacteria as well as how to streak plates so we can get single colonies. These plates will be shipped off to Genewiz for sequencing.

Part 2, March 14 at 7pm: We'll analyze our sequencing results using some basic bioinformatic techniques in order to determine the species of our specimens. You'll learn how to perform and interpret a BLAST search and understand how DNA sequencing works.

*This class is open to members and non-members. Children under 16 are welcome to participate with an adult. The cost of the class is $25 for members and $30 for non-members which covers the cost of materials and sequencing. Members, please pay the $30 through meetup and we will refund you the difference at the event. Please note that the fee covers both sessions.