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Intro to Molecular Biology - Genotyping

At the grand opening there was a lot of interest in introductory classes. Ask and you shall receive.

In this class you will genotype yourself for mutations in the gene TAS2R38 which codes for a bitter-tasting receptor. You will see if your ability to taste phenylthiocarbamide matches up with your genotype. The class is composed of both lecture and hands-on lab work. Students will get experience with the following lab techniques:
- Pipetting
- DNA extraction
- PCR
- Restriction digestion
- Pouring and loading gels
- Gel electrophoresis
If this sounds fun then sign up for the class.

More details:
Limit: 10 people
Cost: $100
Length: approx. 6 hours

Right now I'm limiting it to 10 people but we will be doing this class again shortly. I hope to run this class monthly or bimonthly depending on the demand and the feedback I get.

Also, I should mention that this class is experimental. The lecture content, experimental methods, length, cost, etc are all potentially variable. So please please please give me feedback so I can make it better in the future.

Also, our Paypal address is [masked]. If you're paying by Paypal, please pay ahead of time so I can buy all the materials for the class. You can use the link on the bottom of this (http://www.biohackers.la/genotypeclass) page if you want or just send the money to our email address. We can also except cash and checks made out to "Los Angeles Biohackers" the day of the event.

***Please Note this is Mother's Day so if you already have plans then you're out of luck until next time. And don't forget to call your mom :)

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  • Jonathan

    How and if you pipette could affect outcome in bio protocols.

    PLOS http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0062325

    Dispensing Processes Impact Apparent Biological Activity as Determined by Computational and Statistical Analyses

    Dispensing and dilution processes may profoundly influence estimates of biological activity of compounds. Published data show Ephrin type-B receptor 4 IC50 values obtained via tip-based serial dilution and dispensing versus acoustic dispensing with direct dilution differ by orders of magnitude with no correlation or ranking of datasets. We generated computational 3D pharmacophores based on data derived by both acoustic and tip-based transfer. The computed pharmacophores differ significantly depending upon dispensing and dilution methods. . The acoustic dispensing-derived pharmacophore correctly identified active compounds in a subsequent test set ** where the tip-based method failed. **

    May 13, 2013

  • Roninlaw

    It was awesome. A practical hands on laboratory exercise. The instructor was outstanding.

    May 13, 2013

  • Barbara K.

    Fun, refreshing, interesting.

    May 12, 2013

  • Metro P.

    That was the best meetup ever.

    May 12, 2013

  • Tony

    Excellent class/workshop!!!

    May 12, 2013

  • Lisa

    I just joined the group. I can't make it to this event, but am looking forward to the next one...

    May 10, 2013

  • Jen S.

    This looks so awesome! I find out today if I am able to come, and I hope I can!

    May 8, 2013

  • A former member
    A former member

    Can't make it to this one. I will try for the next one.

    April 30, 2013

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