Past Meetup

Bio-Bootcamp! Two Days of Hands-On Synthetic Biology!

This Meetup is past

7 people went

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Details

LIMITED TO THE FIRST 25 PEOPLE WHO REGISTER!

The best deal in synthetic biology! $50 for two days of intensive hands-on instruction in the latest biological techniques and lab practices.

Whether you're ramping up with us for iGEM or are just interested in learning these biology skills and protocols, join us for our two-day bio bootcamp, July 30th and 31st from 10am to 5pm both days! No specific background or skills required!

Register here: http://goo.gl/forms/lpN7YYe6ui4xJhZf1 . REGISTRATION IS REQUIRED TO ATTEND THIS EVENT!

Skills you’ll learn:

Gibson Cloning
Cell Transformations
DNA mini-preps
Restriction Digests
Gel Electrophoresis
Isolate your own DNA
PCR amplification
DNA sequencing

Detailed Agenda:

First, we will be picking a gene of interest. We will then be cloning our gene into a plasmid, using Gibson Assembly. We can then transform bacteria to express our plasmid-gene construct. The bacteria will propagate, making tons of our plasmid. We will then isolate the plasmid and confirm proper cloning via restriction digest. Second, we will be isolating our own genomic DNA. We will then PCR amplify a region of interest (whatever gene we decide to amplify), using primers that we design. We will then send these samples to ETON Bioscience and they will perform Sanger Sequencing.

This meetup is solely intended for the educational purpose of learning molecular biology concepts and techniques and is not intended to diagnose, treat, cure, or prevent any disease.