Past Meetup

Journal Club: Enzymatic assembly of DNA molecules up to several 100 kilobases

This Meetup is past

5 people went

Every week on Wednesday

MakerLabs.com

780 East Cordova · Vancouver, BC

How to find us

This Wednesday we will meet at our biolab at MakerLabs. At the front entrance just say you are going upstairs to OSN and take the east stairs up, we are right around the corner at the top to the right.

Location image of event venue

Details

Journal Club is an opportunity for citizen scientists to explore and discuss a scientific paper found in a research journal. Over the years participants in the OSN Journal Clubs have had lively discussions on the science and technology behind the papers. These are pitched to anyone who has an interest and attendance is free. Please come on time!

Abstract: This paper describes an isothermal, single-reaction method for assembling multiple overlapping DNA molecules by the concerted action of a 5¢ exonuclease, a DNA polymerase and a DNA ligase. First we recessed DNA fragments, yielding single-stranded DNA overhangs that specifically annealed, and then covalently joined them. This assembly method can be used to seamlessly construct synthetic and natural genes, genetic pathways and entire genomes, and could be a useful molecular engineering tool.

http://www.academia.edu/6310878/Enzymatic_assembly_of_DNA_molecules_up_to_several_hundred_kilobases

Why I chose this article: Scott gave the suggestion for talking about a keystone article on the Gibson assembly process, which is the go-to method for creating longer stretches of DNA containing specific functional sequences. This revolutionizes gene editing as it allowed engineers to create much larger DNA molecules from scratch, instead of relying on template organism sequences where genes could only be inserted into a frame.

Biography: Ian is currently a student in his final year pursuing a Joint Honours of biotechnology degree at the University of British Columbia. He was a prior intern at the Open Science Networks, and conducted an independent research project under the department of Microbiology and Immunology in fungal-bacterial interactions. Ian has presented and has led discussions in the past about his and other work, and is currently the organizer for chairing journal club sessions.
Conceived in the spirit of the VHS's open nights, Open Lab Wednesdays are an opportunity to connect with the lab, meet the people working on project, bring whatever your working on, whatever ideas you have, to an informal meet'n'greet at our lab. Member's are often present doing PCR, pipetting enzymes, having fun. Some Open Lab Wednesdays will have a speaker present a topic of interest to the community or a workshop/demonstration. If you would like to do a talk please email us your ideas at info at opensciencenet dot com. Don't forget to signup on our mailing list (http://www.opensciencenet.org).

There are no requirements for these meetups, just come yourself, meet anyone and everyone involved, learn about potential projects, propose your own, and see our lab as it has evolved until now!

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