À propos de nous
Become a member of the first community biology lab in the East Bay. A place to explore, learn, work on fun projects and tinker with biology. Open to biotech professionals, hobbyists, and citizen scientists. No bio background needed! Be part of our community of creative thinkers, hackers and mad scientists!
Become a member! https://www.counterculturelabs.org/join
You can also check us out on Instagram!
If you have any questions you can reach us by filling out our contact form
Want to support youth education and the next generation of citizen scientists? Make a donation to fund our educational programs!
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![CCL Working Meeting and Drop-In [ONLINE]](https://secure.meetupstatic.com/photos/event/3/4/a/e/highres_460273486.jpeg)
CCL Working Meeting and Drop-In [ONLINE]
·En ligneEn ligneCome join us to help build YOUR lab. CCL is a 100% unpaid-volunteer-after-hours-run labor of love. There is no "staff" - just a bunch of people figuring out together what needs to get done. Help pitch in to build something amazing together!
Drop in to our weekly online meeting to meet us and talk about your project idea! We also spend this time as volunteers to help keep the lab running. RSVP on Meetup for Zoom link.
Can't attend but still want to get in touch? You can reach us via our contact form!
Want to support youth education and the next generation of citizen scientists? Make a donation to fund our educational programs and community projects!
2 participants
Weekly Pandemic Chat
·En ligneEn ligneThis is a weekly zoom call hosted by the Counter Culture Labs and BioCurious communities, for anyone interested in reviewing the week's science and news on COVID-19. Plus guest stars monkeypox, Ebola, RSV and influenza. We welcome your questions and are happy to discuss any articles or news you may have found.
Please sign up for this free event on Meetup to see the Zoom connection info.
Want to support youth education and the next generation of citizen scientists? Make a donation to fund our educational programs and community projects!
2 participants- $600.00
![CRISPR Gene Editing Bootcamp Hands-On [Limited Edition]](https://secure.meetupstatic.com/photos/event/c/c/c/b/highres_533512427.jpeg)
CRISPR Gene Editing Bootcamp Hands-On [Limited Edition]
Counter Culture Labs, 549 48th St, Oakland, CA, US4 spots left
Join us at Counter Culture Labs for a 2-day bootcamp where you will learn the fundamentals of CRISPR-Cas9 and make precision gene edits in E. coli.
CRISPR-Cas9 is a precision genetic engineering tool where guide RNA directs a Cas9 enzyme to a specific target to make a double-stranded break which can be repaired with template DNA. Not only will we genetically engineer microbes with CRISPR but also design our own edits and verify transformations with molecular biology techniques, including PCR and gel electrophoresis.Program Details:
April 25 & 26 weekend from 10am - 4:30pm daily
6 spots total - RSVP TodayDay 1
- History of CRISPR
- Plasmid DNA and inserting DNA into microbes
- Evolution of competence (techniques: heat shock, chemical, electroporation)
- Preparing electrocompetent cells and performing electroporation
- Selection vs screening and plating transformants
- Electronic lab notebook
Day 2
- Observing phenotypes
- Genotyping tools for verifying edits
- PCR to amplify transformant DNA
- Gel electrophoresis and sequencing
- Generating your own Cas9 sgRNA and template
- DNA design for inserting fluorescent proteins into microbes
- Advanced CRISPR applications and technologies
The workshop fee is $600. Venmo @ccl-plantbio. Last four digits phone # confirmation: 6986. Message the organizer on Meetup if you need an alternate payment method. This covers instruction, the materials and equipment used in the class.
***Required lab attire for safety: Long pants and closed-toed shoes.*** Please bring a laptop so that you can participate in the DNA design lessons.
Unable to attend? Interested in other classes like plant genetic engineering? Join our mailing list to hear about upcoming Bay Area workshops!
About The Instructors
Jason Liu PhD is a translational biology scientist and manager specializing in leading teams for therapeutic, programmable large DNA integrations. He has 15+ years of expertise in accelerating novel CRISPR technologies to human cells, molecular biology, cell/strain engineering, and high throughput and NGS assay development.Anthony Neil Tan is a UC Berkeley trained bioengineer whose projects center on enhancing high-value plants through genetic engineering. His past work ranges from designing a plant growth chamber that won a national NASA contest to developing new plant genetic engineering tools at the U.S. Department of Energy's Joint BioEnergy Institute.
No refunds can be issued.3 participants
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