Well, GFP is toxic, so sometimes cells do kick it out. Pick a couple colonies and extract the DNA!
--
William Gunn | Head of Academic Outreach | [masked]
http://www.mendeley.com/profiles/william-gunn
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On Oct 5,[masked]:48 PM, "Patrik" <[address removed]> wrote:
> Woohoo- thanks guys! Looks like everyone got at least one colony, and
> nobody got anything without antibiotic selection.
>
> I noticed some non-glowing colonies on some of the plates - any idea
> what those might be? E.coli with active resistance gene but defective
> GFP? Naturally resistant bacteria from environmental contamination?
> Fungal spores?
>
> Patrik
>
> On Wed, Oct 5, 2011 at 6:30 PM, Tito Jankowski <[address removed]> wrote:
>> Hi everyone,
>> Ryan Huber and I checked up on the GFP cells last week at BioCurious. Check
>> out your GLOWING CELLS here! And thank Ryan for taking all the pictures with
>> the UV light!
>>
https://picasaweb.google.com/106915028995795376365/GFPExperimentAtBioCurious?authkey=Gv1sRgCKGtm_WOw-fdqQE
>> Tito
>>
>>
>>
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