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Upcoming events (5+)
A fundamental technique for modern biotechnology is growing and maintaining cells outside of the multicellular organism. Cells need a variety of nutrients and need to be kept sterile to protect the cells from environmental bacteria and fungi. This class is an introduction to basic cell culture techniques with lab work. We will cover laboratory setup, safety and aseptic working techniques. Using the A549 cell line, you will get hands-on experiences in: - cell passaging - medium change - cell counting This class will be a pre-requisite for future cell biology classes (upcoming classes will include transfection, stem cells, and more) that will teach you to genetically modify these cell lines. 12:00 Welcome & Introduction 12:15 talk "Introduction Cell Culture" 01:15 coffee break 01:30 talk "Hands-On description" 02:00 - 5:00pm Hands-on session Let’s step into the fascinating world of cell culture! Cost: $100/person, $75 for members (please email [masked] to register as a member). Class limited to 8 attendees. The class is open to high school students and anyone who wants experience in basic lab techniques. Please wear closed toed shoes, tie back long hair, and bring a notebook to take notes. Instructor: Ulrike Pflückhahn
Every other Wednesday at 7:00pm Meetings will be hosted at BioCurious. Cuttle Wranglin': Studying the RNA Editing capabilities of the dwarf cuttlefish along with their basic biology. This is an ongoing meeting of a group of citizen scientists that aim to dive deep into the genome of Sepia bandensis (dwarf cuttlefish). Our plan is to perform genome and transcriptome analysis of this species, ultimately leading to the identification of highly edited RNA transcripts. We hope to leave with a clear picture of experimental design but we hope to find a correlation between changing environmental conditions and RNA editing capabilities. For added fun, the edited proteins can be expressed and analyzed for structure and function! In addition to studing the genome of the cuttlefish, we are exploring their basic biology and how to create cell lines. THIS COMMUNITY PROJECT IS OPEN TO: all community members seeking to dive deep into genome/transcriptome analysis. Attendees must be middle school aged or older. If you have bioinformatics or cuttle wranglin' experience or experience setting up and managing a reef tank, your participation would be greatly valued! Zoom Meeting info --> Join from PC, Mac, Linux, iOS or Android: https://zoom.us/j/484560210 Or iPhone one-tap : US: [masked],,[masked]# or [masked],,[masked]# Or Telephone: Dial(for higher quality, dial a number based on your current location): US: [masked] or [masked] or [masked] Meeting ID:[masked] International numbers available: https://zoom.us/zoomconference?m=venU-3adqsFd6cIg6DNMKc0nU8MYS4Y0
Come join our ongoing BioPrinter community project! Did you know you can print live cells from an inkjet printer? Companies like Organovo are developing ways to 3D print human tissues and organs. But the basic technologies are so accessible that we wanted to play around with them ourselves. We've built our own functioning bioprinter from a couple of old CD drives, an inkjet cartridge, and an Arduino. We probably won't be printing human organs any time soon, but how about printing a leaf from plant cells? Or add a BlueRay laser to turn it into a miniature laser cutter to print "lab-on-a-chip" microfluidic devices. The possibilities are endless - it all depends where *you* want to take it! Our community projects are open to anyone, and are driven entirely by whoever wants to show up and participate. This is a great opportunity to come check out BioCurious, and jump into some of the projects going on. This project has something for everyone, whether it's hardware hacking. programming, Arduinos, microfluidics, synthetic biology, plant biology, cell culturing, tissue engineering - you name it! Everyone has something to learn, or something to teach. Check out the progress we've made so far in our BioPrinter instructable: http://www.instructables.com/id/DIY-BioPrinter/ We'll try to give a brief intro to the project at the start of the meeting for any new visitors. This is a hacking meeting, so bring your favorite tools: laptop, camera, soldering iron etc. Younger kids must be accompanied by an adult, thanks. The fee for this event is by donation. BioCurious exists because of memberships, donations, and volunteer contributions. Donations can be made via Paypal at [masked] . If you cannot join us in person, feel free to connect online: Please click this URL to start or join. https://zoom.us/j/820128495 Or, go to https://zoom.us/join and enter meeting ID:[masked] Or join from dial-in phone line: Dial: +1 (415)[masked] or +1 (646)[masked] Meeting ID:[masked]
Welcome to our new community project: Kombucha Genomics! For all you fermented foods lovers out there, this is the project for you. Over the course of the next couple months, we'll go through all the steps to isolate and identify a variety of bacterial and yeast strains from kombucha or other fermented foods (bring your own), starting with the basic culturing and progressing all the way through DNA barcoding, genome and microbiome sequencing, systems biology, synthetic biology, and more! Kombucha (https://en.wikipedia.org/wiki/Kombucha) is a fermented tea with a very complex symbiotic culture of bacteria and yeasts (SCOBY) (https://en.wikipedia.org/wiki/SCOBY), composed of dozens of different species. It's a health food; it's a tasty fizzy caffeinated beverage; it even makes vegan leather (http://www.ecouterre.com/u-k-designer-grows-an-entire-wardrobe-from-tea-fermenting-bacteria/) - what's not to like! We hope to run this project in collaboration with our friends at Counter Culture Labs and other community labs around the world. You can join us remotely over Zoom at https://zoom.us/j/820128495 Or, go to https://zoom.us/join and enter meeting ID:[masked] . Or to call in by phone, dial: +1 (415)[masked] or +1 (646)[masked] Meeting ID:[masked] Please bring yourself, a laptop or other internet enabled device to take notes and do research is always useful.