Biotech Boot Camp 101A: PCR Basics – Techniques and Applications

Biotech 101 is part of our Biotech Boot Camp series.

Biotechnology 101A (PCR) and 101B (Cloning & Sequencing) will teach you what you need to know to do a small biotech project of your own or introduce you to basic techniques we “lab rats” use during the day!

This is a 2-part class offered consecutive Saturdays

February 18, 2012 from 9am to 1pm

February 25, 2012 from 9am to 1pm

Sign up soon, only 16 spots are open!

Biotech 101A is a 2-part class that will introduce you to PCR (Polymerase Chain Reaction). PCR is a fundamental technique of modern biotechnology - developed here in the Bay Area by Nobel Laureate Kary Mullis. You will have the opportunity to run and analyze your own genomic DNA using standard PCR techniques. You will be looking at your genotype for an ALU element. The study of ALU elements has allowed us to understand evolution and the inheritance of specific populations.

This class will introduce you to PCR Applications including: DNA fingerprinting, diagnostic medicine, genome mapping, and evolutionary relationship determination! No prior experience is necessary. We will teach you the required techniques and necessary background during the workshop!

This workshop includes:

• Reagents for isolating your individual DNA, performing PCR and analysis of your PCR products

• Handouts of Presentation Slides, Key Concepts from lectures and “How To” Protocol Steps

• Lecture and lab on PCR Reaction Components and Technology, Agarose Gel Electrophoresis, on Analysis of PCR Products

• Introduction to PCR Applications, Primer Design, PCR Variations and PCR Optimization

Pricing:

Sign up by Friday 2/17/12 and pay only $100.00 for a 2-part, 8-hour class* Price at the door or day of class is $150.00

• • For non-members, class fee can be applied towards Biocurious membership