Biotech Boot Camp 101B: Cloning and Sequencing Techniques

Biotech 101 is part of our Biotech Boot Camp series. Biotechnology 101A (PCR) and 101B (Cloning & Sequencing) will teach you what you need to know to do a small biotech project of your own or introduce you to basic techniques we “lab rats” use during the day! Biotech 101B is a 3-part class that will introduce you to the fundamental biotechnology techniques of Cloning and Sequencing. Cloning and Sequencing applications include production of recombinant proteins (such as insulin), creation of genetically modified organisms (GMO, such as transgenic mice and plants), and gene therapy! In this workshop, you insert yeast DNA into E.coli using the techniques of ligation and bacterial transformation. We will subsequently submit our sample of E.coli DNA to a lab for sequencing. equence the inserted plant DNA and query the public databases to investigate which gene or DNA fragment was inserted into bacteria. No previous experience is necessary. We will teach you the required techniques and necessary background during the workshop!

Workshop includes:

Reagents for performing Ligation, Making a Library, Bacterial Transformation

Cloning and Sequencing of yeast DNA

Handouts of Presentation Slides, Key Concepts from lectures and “How To” Protocol Steps/ Lecture and lab on fundamental techniques

Introduction to Cloning and Sequencing Applications.

Dates and Pricing

This is a 3-part class offered consecutive Wednesdays

February 15, 2012 from 6:30 PM to 9:00 PM

February 22, 2012 from 6:30 PM to 9:00 PM

February 29 from 6:30 PM to 9:00 PM

Sign up soon, there are only 16 spots open! Young scientists under 16 years old must be accompanied by an adult.

Sign up by 2/14/12 and pay only $100.00 for a 3-part, 8 hour class* On the day of the first class (2/15/12), the price is $150.00.

• • Class fee can be applied towards Biocurious membership