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DIY Molecular Cloning: Plasmid Construction

  • Apr 1, 2014 · 7:00 PM
  • Ahnon's

So, you survived a introductory lecture and a banana cloning experiment. Now, let's get serious about Cloning.

I've spent the last couple of days revamping the class so we can perform high quality molecular bio experiments. I've created a syllabus that can be found at

Within the syllabus is the course budget. That budget can be validated online if you desire. I would ask that if you decide to attend the class, that you pitch in with the cost of reagents.

You will also find the class dates and topics to be discussed with assigned reading materials. We will cover specific topics each class (Every other Tuesday starting March 4, 2014) and perform corresponding laboratory work in the class. Lectures will be more focused and occur before lab work or during experimental wait times.

Today's topic will be the Polymerase Chain Reaction (PCR) - how to multiply a piece of DNA to a high eenough level you can do something with it (like sequencing, or inserting it somewhere else)

I intend to have you leave the class in June with a thorough understanding of how to perform your own cloning experiments!

Join or login to comment.

  • Rashmi

    Hi Guys,
    Unfortunately last minute issues came up at work and I won't be able to get to the lab in time...sorry about having to cancel last minute! I was looking forward this lab =(

    April 1, 2014

  • Jasmine

    Hi Guys. I have a work event at 4:30 and afterwards I know I am going to be ready to go home. BUT I wanted to invite you to that work event. I am sponsoring a happy hour at Prize Fighter, in Emeryville. The drinks are 100% on me (and 3 other nice vendors). It is chill and a good way to meet academic and nonacademic science people and most attendees really enjoy themselves. If you don't want to be tipsy before your cloning, I totally understand.

    March 31, 2014

  • Naomi F.

    Is this happening at Sudo Room or Ahnon's?

    March 31, 2014

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