Sunday DIYBIO Workshop at the Hacklab!

This Sunday January 26th, DIYBio.to welcomes you to a hands-on PCR workshop! No experience necessary. This is meant to be a fun and informative exploration of PCR, which is the cornerstore tech of molecular biology.

Course Description: Basic PCR protocol for Standard PCR and some Real-time qPCR. Learn to micropipette and set up PCR reactions.

Expectations and goals: Learn what PCR is and how to perform it.
DNA amplification using short DNA oligonucleotides, an enzyme to copy the DNA strands, and amplify them by attaching the nucleotides that correspond to the opposite strand. Given a set of primers that is well defined for the specific target, the method is fairly straightforward on paper, but requires attention to detail in practice. Using a Taq mix, the primers and mix are added together. Making Taq mixes from scratch is also possible, and requires utmost care in the preparation – keeping all DNA samples away from the preparation site. The next step is adding the template or test sample, and setting up the PCR machine. Afterwards, we will also run a gel.

This class will be limited to a select few people, as we can only train a small number of people at once. We also welcome you to chat about biology, biotech, scientific approaches. While this is a low-cost event, donations to keep our projects well supplied are welcome!

At [DIYBio.to](https://diybio.to/), our mission is to bridge the gap between biology and the community, fostering a reciprocal relationship where people can reconnect with the wonders of biology. Operating in a neutral, open, and not-for-profit environment, we are dedicated to making biology accessible.

Who we are: Our community comprises biologists and enthusiasts, ranging from individuals with a keen interest to PhD-level scientists and engineers.

Code of Ethics: We adhere to the DIYBio code of ethics, as outlined on diybio.org.

Membership: If you share our passion and vision, we invite you to become a member. Come visit us, and let's engage in a conversation about DIYBio.to. Your journey into the world of DIY biology awaits!

Exceptional circumstances where the course may be postponed = extreme weather, illness.
Payment through Hecim (like interac) or cash at the event.