Saturday DIYBIO Workshop at the Library! NB: Registration required.

Saturday October 4th, DIYBio.to welcomes you to an offsite hands-on PCR workshop! No experience necessary. This is meant to be a fun and informative exploration of PCR, which is the cornerstone tech of molecular biology.

Course Description: Basic PCR protocol for Standard PCR and Real-time qPCR. Learn to micropipette, design primers and set up PCR reactions.

Expectations and goals: Learn what PCR is and how to perform it.
DNA amplification using short DNA oligonucleotides, an enzyme to copy the DNA strands, and amplify them by attaching the nucleotides that correspond to the opposite strand. Given a set of primers that is well defined for the specific target, the method is fairly straightforward on paper, but requires attention to detail in practice. Using a Taq mix, the primers and mix are added together. Making Taq mixes from scratch is also possible, and requires utmost care in preparation – keeping DNA samples away from the preparation site. The next step is adding the template or test sample, and setting up the PCR cycler. Also, we will run an agarose gel of PCR reactions.

This class will be limited to a small number of people, to ensure that we can train everyone well. We also welcome you to chat about biology, biotech, scientific approaches. While this is a free event, DONATIONS to help keep our projects supported are most welcome!

At [DIYBio.to](https://diybio.to/), our mission is to bridge the gap between biology and the community, fostering a reciprocal relationship where people can reconnect with the wonders of biology. Operating in a neutral, open, and not-for-profit environment, we are dedicated to making biology accessible.

Who we are: Our community comprises biologists and enthusiasts, ranging from individuals with a keen interest to PhD-level scientists and engineers.

Code of Ethics: We adhere to the DIYBio code of ethics, as outlined on diybio.org.

Membership: If you share our passion and vision, we invite you to become a member. Come visit us, and let's engage in a conversation about DIYBio.to. Your journey into the world of DIY biology awaits!

exceptional circumstances where the course may be postponed = extreme weather, illness