The use of nucleotide sequence variations to investigate evolutionary relationships is not a new concept. DNA barcoding is a method that uses a short genetic marker in an organism's DNA to identify it as belonging to a particular species. In 2003, Paul D.N. Hebert from the University of Guelph, Ontario, Canada, proposed the compilation of a public library of DNA barcodes that would be linked to named specimens. This library would "provide a new master key for identifying species, one whose power will rise with increased taxon coverage and with faster, cheaper sequencing".
We will be discussing the DNA barcoding technique and how it can be applied to meta-barcoding the microorganisms in Kombucha SCOPYs.
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