Past Meetup

TriDIYBio Third Sunday Meeting

This Meetup is past

3 people went

Every 3rd Sunday of the month

Splat Space

800 N. Mangum · Durham, NC

How to find us

We're on the NE corner of N. Mangum and E. Corporation Sts. just N of downtown Durham. Enter at the Corporation St. gate, then park and enter Splat at the N side of the building. (919) 704-4225.

Location image of event venue

Details

An overview of the basics of Illumina next generation sequencing will be presented along with a basic hands-on QC analysis of a dataset. A brief introduction to QIIME, a widely used 16S microbiome sequence analysis tool will be given. Attendees may want to install one or both the software free packages listed below if they want to try some hands-on bioinformatics analysis. This is all to get TriDIYBio members more up to speed for the microbiome analysis of various Mudwatts that we are planning.

Fastqc: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/
QIIME2: https://docs.qiime2.org/2018.11/install/native/

Versions are available for major OS (Mac, Linux, Windows)

We may get a little ways into a QIIME tutorial, depending on time: https://docs.qiime2.org/2018.11/tutorials/atacama-soils/

If not once QIIME is installed any user should be able to run the tutorial on their own time.