Advanced CRISPR Gene Editing Bootcamp (6 spots)

Advanced Molecular Biology: CRISPR Gene Editing 3-day Bootcamp

Join us at BioCurious for a 3-day bootcamp where you will learn the fundamentals of CRISPR-Cas9 and make precision genome edits in E. coli to confer antibiotic resistance. This program is open to students and has a limited capacity of 6 seats.

CRISPR-Cas9 is a precision genetic engineering tool where guide RNA directs a Cas9 enzyme to a specific genomic target to make a double-stranded break which will be repaired with template DNA. Not only will we genetically engineer microbes with CRISPR but also design our own edits and verify transformations with molecular biology techniques, including PCR and gel electrophoresis.

Program Details
January 17, 18, 19 from 10am - 4:30pm daily (Jan 19 Monday is MLK Day - no school)
$950 standard admission New Year's Exclusive: Book by January 3rd and save $150 on your RSVP! Register for just $800 (regularly $950)
Venmo @ccl-plantbio. Last four digits phone # confirmation: 6986. Message the organizer on Meetup if you need an alternate payment method. This covers instruction and the materials and equipment used in the class.

Day 1

• History of CRISPR
• Plasmid DNA and inserting DNA into microbes
• Evolution of competence (techniques: heat shock, electroporation)
• Preparing electrocompetent cells and performing electroporation
• Selection vs screening and plating transformants

Day 2

• Observing phenotypes
• Genotyping tools for verifying edits
• PCR to amplify transformant DNA
• Using counterselection

Day 3

• Gel electrophoresis and sequencing
• Generating your own Cas9 sgRNA and template
• DNA design for inserting fluorescent proteins into microbes
• Electronic lab notebook

Required lab attire for safety: Long pants and closed-toed shoes.
​
Unable to attend? Interested in other classes like plant genetic engineering? Join our mailing list to hear about upcoming Bay Area workshops!

About The Instructors
Jason Liu PhD is a translational biology scientist and manager specializing in leading teams for therapeutic, programmable large DNA integrations. He has 15+ years of expertise in accelerating novel CRISPR technologies to human cells, molecular biology, cell/strain engineering, and high throughput and NGS assay development.

Anthony Neil Tan is a UC Berkeley trained bioengineer whose projects center on enhancing high-value plants through genetic engineering. His past work ranges from designing a plant growth chamber that won a national NASA contest to developing new plant genetic engineering tools at the U.S. Department of Energy's Joint BioEnergy Institute.